Metabolomics Platform

The Metabolomics Platform is specialized in developing and applying mass spectrometry-based comprehensive analysis methods of small molecules in complex biological samples. The Metabolomics Platform supports a wide range of cutting-edge metabolomic approaches for several sample types, including mammalian cells, bacteria, yeast, body fluids (e.g.cerebrospinal fluid, plasma, serum, urine), soft tissues (e.g.brain, liver, lung, heart, kidney) and others (e.g.Dried Blood Spots).

However, to address the needs of researchers in this fast changing field, the Metabolomics Platform is continuously developing new analytical (targeted/untargeted) and tailor-made methods to address cutting-edge biological and biomedical questions. Furthermore, the Metabolomics Platform can provide a comprehensive set of tools for a reliable data analysis based on a statistical evaluation. Moreover, the highly competitive price structure enables Luxembourg researchers to obtain fast, accurate and complete data generation in order to successfully pursue their research goals.

Vision

  • Combined analytical service and research facility that supports metabolism (small molecules)-related research in Luxembourg
  • Directly involved in research cooperation to provide optimal analytical output
  • Keeps up with latest developments in the Metabolomics field
  • Method development or method optimization: Selectivity, Sensitivity, Speed

Services

  • Gas Chromatography – Mass Spectrometry (GC-MS)
  • Liquid Chromatography – Mass Spectrometry (LC-MS)
  • Access to equipment and protocols for sample preparation, e.g.
    • Liquid-Liquid Extraction
    • Solid Phase Extraction
    • Evaporation
    • Filtration
    • Sonication
    • Lyophilization
    • Gas Phase Extraction
    • Automated Sample Preparation
  • Support and advise in experimental design (mass spectrometry-based study)

Instrumentation

The largely variable and highly dynamic profiles of metabolites pose a challenge in research, and single analytical techniques are insufficient for a comprehensive metabolomic analysis. Therefore, the LCSB has built up a reliable analytical platform based on both GC-MS and LC-MS to strategically address this complexity. The Metabolomics Platform currently hosts two GC–MS instruments served by multi-functional sample preparation robots, and three LC–MS systems. In addition, the platform offers liquid chromatography analysis services with an HPLC and fast absolute quantitative measurements of more abundant metabolites obtained by a Biochemistry Analyzer.

  • Agilent 7890B GC – Agilent 5977A MSD (+ Gerstel MPS-PrepStation)
  • Agilent 7890A GC – Agilent 5975C MSD (+ Gerstel MPS-PrepStation)
  • Agilent 1290 LC – Agilent 6560 Ion Mobility Q-TOF
  • Thermo Ultimate 3000 LC – Thermo QExactive
  • Shimadzu Nexera LC – Sciex API4000 QTRAP
  • YSI Biochemistry Analyzer 2950D

Contact

The Metabolomics Platform is part of the Enzymology & Metabolism Group (headed by Dr. Carole Linster). For any inquiries or questions regarding methods/instruments, sample processing, SOPs, and prices, please contact:

Dr. Christian Jäger

Email: metabolomics.platform [at] uni.lu